**Job Description**
This research support post is located in the Mishto lab within the Centre for Inflammation Biology and Cancer Immunology (CIBCI) at King’s College London. The primary aim of the role is to identify and validate HLA-I epitopes, including the discovery of noncanonical (mutated) epitopes relevant in cancer immunotherapy. The successful candidate will also be responsible for isolating CD8+ T cell clones specific for HLA-I-restricted neoepitopes, cloning their TCRs, and investigating neoepitope antigenicity in peripheral blood of cancer patients.
**Skills & Abilities**
• Experience of mammalian cell culture (both primary and cell lines)
• Excellent knowledge and understanding of cell biology, molecular immunology, antigen presentation, T and B cell biology
• Documented experience in TCR isolation and cloning in vectors
• Successful experience in identification of noncanonical epitope candidates in multi-omics
• Documented experience in in vitro digestion with purified proteasomes and analysis of mass spectrometry RAW files
• Documented experience in peptide elution from MHC-I complexes
• Evidence of active collaboration with dry lab and co-development of algorithm for the prediction of epitopes (Desirable)
**Qualifications**
Required Degree(s) in:
• Cell Biology
• Molecular Biology
• Immunology
• A related subject
**Experience**
Other:
• Detailed knowledge of applied cellular and molecular biology and immunobiology, biochemistry and cloning would be highly advantageous.
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